The study of antibacterial properties of NiO thin film using Sol-gel synthesis

Nickel oxide thin film of various preferred orientations was deposited using sol-gel method using solvents including methanol. The resulting film was analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), contact angle measurement, and ultraviolet spectroscopy (UV-vis). Antibacterial activities of nickel oxide-based film against gram-negative bacteria (Escherichia coli) has been evaluated using drop test method under UV light irradiation (photocatalyst) and dark conditions (catalytic). The antimicrobial activity of the film was determined through counting the number of colonies on each plate which was reported as colony-forming unit (CFU) per milliliter. Results showed that NiO has high photocatalyst and catalytic activation efficiency against Escherichia coli.